Ascentis express f5 2 µm 15 cm 3,0 mm

Ascentis Express 2.0 μm F5 is a high-speed, UHPLC column based on a Fused-Core®particle design. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.4-micron thick porous shell and the small overall particle size of 2-microns. The bonded, endcapped, dimethylpentafluorophenylpropyl stationary phase of Ascentis Express 2.0 μm F5 provides a stable, reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming p-p and mildly steric interactions, F5phases also retain compounds by polar interactions. Having polarr and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis Express 2.0 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.