Bleu alcian ph 2,5 mowry 1 l

Alcian dyes belong to cuprophthalocyanine group, they bind polyanions of acid mucopolysaccharides
through saline bounds.
In the pH 2.5 method, a sodium tetraborate solution transforms the dye into Monastral blue pigment,
which is insoluble and can therefore be further manipulated without spreading in the tissue (Alcian bluePAS).
Alcian blue reacts with polyanions whose components are sulphuric and carboxylic radicals (phosphate
radicals of nucleic acids do not react). As result, acid mucins only are stained.