FOXP3 Fix/Perm Buffer Set 100 tests

BioLegend's FOXP3 Fix/Perm Buffer Set has been specially formulated for intracellular staining FOXP3 with minimum effect on the surface fluorochrome staining. It contains one 30 ml 4X concentrated FOXP3 Fix/Perm buffer (Cat. No. 421401) and one 25 ml 10X concentrated FOXP3 Perm buffer (Cat. No. 421402). The FOXP3 Fix/Perm buffer(4X) must be freshly diluted to 1X working solution with PBS (10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2) prior to staining procedures. e.g. Dilute one (1) part FOXP3 Fix/Perm buffer(4X) to three (3) parts PBS. The FOXP3 Perm buffer (10X) should be diluted to 1X working solution with PBS (10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2) prior to staining procedures. e.g. Dilute one (1) part FOXP3 Perm buffer(10X) to nine (9) parts PBS. Warning: The FOXP3 Buffer Set can have a deleterious effect on tandem fluorophores (particularly APC/Cy7) in a multicolor assay, causing an increase in compensation into the APC channel. If your panel contains a surface stain with an antibody conjugated to a tandem fluorophore, consider using the True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) instead, which provides a clearer resolution of FoxP3 as well as other nuclear transcription factors. Please refer to the data provided here. Caution: The FOXP3 Fix/Perm buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contact. ;