Zombie Fixable Viability Sampler Kit

The Zombie dyes in this kit (provided at 100 tests each) are the UV, NIR, Violet, Aqua and Yellow variants. The Zombie live/dead fixable dyes are amine-reactive fluorescent dyes that are impermeant to live cells but permeant to cells with compromised membranes. They irreversibly conjugate to primary amine-containing proteins. Since there is a greater abundance of proteins inside the cell versus the cell surface, dead cells with a compromised membrane stain brighter than live cells with an intact membrane. Thus, they can be used to assess live vs. dead status of mammalian cells. However, titration of the reagent is required to ensure that live cells have minimal to no staining due to this dye on the cell type of interest. It is also important to consider that the accuracy of the live/dead assessment is dependent on the uniformity in size of each cell since larger cells will stain brighter than smaller cells, possibly confusing large cells as dead cells. Zombie live/dead fixable probes are useful for incorporation into multicolor panels, particularly in applications where cells will be fixed and permeabilized prior to analysis. To find the spectra of each of the Zombie dyes offered in this kit, please find them here: https://www.biolegend.com/spectraanalyzer;