HTRF - Acc total - 500 tests 1 Kit

Alternative ELISA et WB, plus simple, plus rapide, très sensible, facilement miniaturisable / automatisable et peu onéreuse.

ACC (Acetyl-CoA carboxylase) is an important regulator of fatty acid metabolism. By catalyzing the carboxylation of acetyl-CoA to malonyl-CoA, ACC serves as a novel biomarker or drug target in diabetes and obesity research. The enzymatic activity of ACC is inhibited by phosphorylation on Ser-79 residue. Like various genes involved in fatty acid regulation, the transcription of ACC genes is tightly regulated by the SREBP1c transcription factor. When activated, this transcription factor binds to the SRE DNA sequence upstream from the ACC gene promoter, and activates its transcription. Thus, the quantitative detection of Total ACC represents a valuable readout of SREBP1c pathway activation. The HTRF Total ACC cellular assay monitors the expression levels of both ACC 1 and ACC2, and can be used as a normalization assay with the HTRF phospho-ACC Ser79 kit. This kit is compatible with the buffers from the phospho-ACC kit, so the same lysate can be used for analyzing both the phosphorylated and the total protein populations. This cell-based assay enables simple yet sensitive and efficient quantification of ACC 1/2, and offers enhanced convenience over ELISA or WB assays.