HTRF - Cereblon total - 500 tests 1 Kit

Alternative ELISA et WB, plus simple, plus rapide, très sensible, facilement miniaturisable / automatisable et peu onéreuse.

The Total Cereblon cellular assay measures the expression level of the E3 ubiquitin ligase Cereblon (CRBN) in cells. Cereblon is a ubiquitously expressed protein which functions as a substrate recognition subunit within the CRL4-CRBN E3 ubiquitin ligase complex, and targets specific proteins for proteolysis through the Ubiquitin Proteasome System (UPS). Cereblon plays a key role in normal limb outgrowth, normal cell proliferation and metabolism, and in memory and learning.  Cereblon is also the primary direct target of the immunomodulatory drugs (IMiDs) Thalidomide, Pomalidomide, and Lenalidomide, that are all FDA approved drugs for the treatment of multiple myeloma. Interestingly, IMiDs have recently been derived into PROTAC molecules in order to direct degradation of undesired proteins by the UPS.