HTRF - Htrf thalidomide - red ligand 1 Kit

Alternative ELISA et WB, plus simple, plus rapide, très sensible, facilement miniaturisable / automatisable et peu onéreuse.

HTRF Thalidomide-Red ligand is primarily intended to perform affinity binding curves to Cereblon protein using HTRF® technology. Cereblon, also known as CRBN, is involved in many biological processes and is closely associated with proliferation, apoptosis, cell metabolism, and the occurrence of many diseases. Cereblon is also one of the two most popular E3 ligases which is recruited by bifunctional Proteolysis-targeting chimeras (PROTACs) to induce ubiquitination and subsequent proteasomal degradation of a targeted protein. Cereblon interacts with several proteins to form the functional E3 ubiquitin ligase complex, in which CRBN functions as a substrate receptor of the E3 ubiquitin ligase complex and targets proteins for proteolysis. HTRF Thalidomide-Red ligand binding assays can be used in association with the HTRF Cereblon binding kit (#PerkinElmer 64BDCRBNPEG/H) for cooperativity binding studies in order to monitor the effect of PROTAC protein substrate on the Kd of HTRF Thalidomide-Red ligand for Cereblon protein, enabling accurate analysis of cooperative effect monitored with the HTRF Cereblon binding kit.