HTRF - Phospho-total protein - blocking reagent (100x)- 2 ml 1 Kit

Alternative ELISA et WB, plus simple, plus rapide, très sensible, facilement miniaturisable / automatisable et peu onéreuse.

Proteins and phosphoprotein may be prone to degradation following cell lysis, e.g. by endogeneous phosphatases. When running a cell-based assay, an appropriate stabilizing and blocking reagent is thus mandatory in order to prevent any remaining enzymatic activity, as well as to garantee protein integrity and quantification.