OneScript RT Mix for qPCR w/gDNAOut 100 réactions

OneScript® RT Mix for qPCR with gDNAOut is an efficient and fast first-strand cDNA synthesis master
mix, suitable for two-step RT-qPCR detection. The 5X OneScript® RT Mix in this product contains all
the relevant reagents required for the reverse transcription reaction. It is only needed to add RNA
template and DNase/RNase-free water to perform the reverse transcription reaction. The protocol is
simple. The OneScript® Reverse Transcriptase in the master mix is a new-generation reverse
transcriptase developed based on M-MuLV Reverse Transcriptase, which has reduced RNase H activity
and improved thermal stability. The reaction temperature can be settled to up to 55°C, which greatly
improves reverse transcription reaction efficiency and tolerance of complex RNA transcripts (high
secondary structures or high GC contents), with higher specificity and higher yield.

The gDNAOut Mix in this product can completely remove residual genomic DNA in RNA samples and
makes the quantitative results more accurate. It is heat sensitive and can be rapidly and irreversibly
inactivated by heating at high temperature. So only sample addition is required, and the reaction can
be carried out in the same tube to remove genomic DNA contamination and to reverse transcribe the
RNA. This cDNA product is specially optimized for qPCR. The primer mix with optimized balance of
Random Primers/Oligo d(T)20VN Primers enables that the cDNA synthesis starts at any region of the
RNA transcript and with the same reverse transcription efficiency, ensuring high authenticity and
reproducibility of qPCR results.

The reverse transcription product is compatible with SYBR® Green and probe method qPCR. According
to the purpose of the experiment, the qPCR reagents can be selected to have high-performance gene
expression analysis.