Pcrbio verifi mix (2x1,25 ml) 100 réactions

PCRBIO VeriFi™ Mix is a convenient high fidelity 2x mix designed for PCR
applications where greater sequence accuracy is required, together with improved PCR success rates of long and
challenging templates.
PCRBIO VeriFi™ Mix contains the engineered and highly processive PCRBIO VeriFi™ Polymerase, developed for fast and versatile high fidelity PCR.
The enzyme is derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity.
Several proprietary mutations significantly improve DNA binding and processivity, resulting in shorter extension times (10-30s/kb), higher yields and the ability to amplify longer and more
difficult targets, including eukaryotic genomic templates in excess of 17.5kb.
The high accuracy and enhanced 3’-5’ exonuclease activity of PCRBIO VeriFi™ Polymerase result in fidelity that is approximately 100 times higher than Taq DNA polymerase. The enzyme is ideally suited to applications where greater accuracy is required, such as cloning, site-directed mutagenesis and
sequencing. PCR products generated with this range of products are blunt ended.
PCRBIO VeriFi™ Mix uses an advanced buffer system including dNTPs, Mg and enhancers enabling high fidelity PCR of a wide range of targets and fragment sizes with minimal or no
optimisation required.