Pluristemdispase-2solution(100 ml)1mg/ml 100 ml

Proteolytic enzymes such as trypsin, collagenase and pronase are commonly used for dispersing tissues and cells. These enzymes, however, often injure the cells, are unstable during incubation, can be heterogeneous and also a source of mycoplasma contamina Optimal passaging technique must be determined by end user and varies depending on cell type and culture conditions. 1. Coat 6-well plates with 1:20 Matrigel coating (1.5 ml per well). Incubate at 2-8ºC overnight or coat at RT for at least 30 minutes

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